ABSTRACT
Purpose To evaluate the effects of double stai-ning of elastic fibers and broad-spectrum keratin and simple e-lastic fiber staining in pleural invasion of lung cancer. Methods The pathological data of lung cancer from June 2015 to July 2016 in Jiangsu Province Hospital were retrospectively analyzed, of which 104 cases presented with suspected pleural invasion by HE staining. Double staining of elastic fibers and broad-spec- trum keratin and simple elastic fiber staining were adopted to compare their effects for pathological analysis on pleural invasion of lung cancer. Results 46 patients with positive pleural inva-sion were reported by simple elastic fiber staining, whereas 63 patients with positive pleural invasion were detected by double staining. The positive rate of double staining was 60. 58% , however the positive rate of simple elastic fiber staining was 44. 23% . The ability of double staining on detection of pleural invasion was significantly higher than simple elastic fiber stai-ning. Furthermore, following pathological analysis revealed that patients with contradictory results using double staining and sim-ple staining were mostly diagnosed by presence of single or sever-al broad-spectrum keratin-positive tumor cells which invaded the pleura. Conclusion Compared with simple elastic fiber stai-ning, double staining of elastic fibers and broad-spectrum keratin combines the advantages of these two methods, which can simul- taneously visualize the pleural and tumor cells, so as to better observe the adjacent relationship between tumor cells and sur-rounding pleura. Double staining of elastic fibers and broad-spectrum keratin is an efficient method for pathological diagnosis and benefit to following clinical treatment of patients with positive pleural invasion of lung cancer.